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transcriptome sequencing analysis rna sequencing  (Novogene)

 
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    Structured Review

    Novogene transcriptome sequencing analysis rna sequencing
    Transcriptome Sequencing Analysis Rna Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+transcriptome+sequencing/rna+sequencing/pm42316183-132-0-12
    Average 86 stars, based on 1 article reviews
    transcriptome sequencing analysis rna sequencing - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Sequencing:

    Article Title: KLF2-dependent transcriptional regulation safeguards the heart against pathological hypertrophy.
    Article Snippet: Background: Our previous single-cell RNA sequencing study in the adult human heart revealed that cardiomyocytes from both the atrium and ventricle display high activities of Krüppel-like factor 2 (KLF2) regulons.. However, the role of the transcription factor KLF2 in cardiomyocyte biology remains largely unexplored.. Methods and results:We employed transverse aortic constriction surgery in male C57BL/6 J mice to develop an in vivo model of cardiac hypertrophy, and generated different in vitro cardiac hypertrophy models in neonatal rat ventricular myocytes and human embryonic stem cell-derived cardiomyocytes.

    Article Title: RRM2 Promotes the Progression of Intrahepatic Cholangiocarcinoma by Inhibiting Ferroptosis Through the HIF-1α Pathway.
    Article Snippet: .. RNA transcriptome sequencing was performed by Beijing Novogene Technology Co. Ltd. ..

    Article Title: Transcriptomics-based analysis reveals the nephrotoxic effects of triphenyltin (TPT) on SD rats by affecting RAS, AQPs and lipid metabolism.
    Article Snippet: Triphenyltin (TPT) is a class of organotin compounds that are extensively used in industry and agriculture.. They have endocrine-disrupting effects and cause severe environmental contamination.. Pollutants may accumulate in the kidneys and cause pathological complications.

    Article Title: Overexpression of a plasmalemma Na + /H + antiporter from the halophyte Nitraria sibirica enhances the salt tolerance of transgenic poplar.
    Article Snippet: The plasmalemma Na/H antiporter Salt Overly Sensitive 1 (SOS1) is responsible for the efflux of Na from the cytoplasm, an important determinant of salt resistance in plants.. In this study, an ortholog of SOS1, referred to as NsSOS1, was cloned from Nitraria sibirica, a typical halophyte that grows in deserts and saline-alkaline land, and its expression and function in regulating the salt tolerance of forest trees were evaluated.. The expression level of NsSOS1 was higher in leaves than in roots and stems of N. sibirica, and its expression was upregulated under salt stress.

    Article Title: Long intergenic non-coding RNA 00467 promotes lung adenocarcinoma proliferation, migration and invasion by binding with EZH2 and repressing HTRA3 expression
    Article Snippet: .. RNA transcriptome sequencing was performed by Novogene Bioinformatics Technology Co. Ltd. ..

    Article Title: X-ray-activated nanoscintillators integrated with tumor-associated neutrophils polarization for improved radiotherapy in metastatic colorectal cancer.
    Article Snippet: Radiotherapy, employing high-energy rays to precisely target and eradicate tumor cells, plays a pivotal role in the treatment of various malignancies.. Despite its therapeutic potential, the effectiveness of radiotherapy is hindered by the tumor’s inherent low radiosensitivity and the immunosuppressive microenvironment.. Here we present an innovative approach that integrates peroxynitrite (ONOO )-mediated radiosensitization with the tumor-associated neutrophils (TANs) polarization for the reversal of immunosuppressive tumor microenvironment (TME), greatly amplifying the potency of radiotherapy.

    other:

    Article Title: Fragile histidine triad (FHIT) suppresses gastric cancer via translational regulation of the epigenetic modifier KDM6B.
    Article Snippet: RNA was isolated from cells or tissues using the TRIzol reagent (Invitrogen).

    Article Title: Fragile histidine triad (FHIT) suppresses gastric cancer via translational regulation of the epigenetic modifier KDM6B
    Article Snippet: The lentiviral vectors for overexpression (pLVX-Puro) and knockdown (pLVX-shRNA1) were obtained from Clontech Laboratories.

    Transgenic Assay:

    Article Title: Overexpression of a plasmalemma Na + /H + antiporter from the halophyte Nitraria sibirica enhances the salt tolerance of transgenic poplar.
    Article Snippet: The plasmalemma Na/H antiporter Salt Overly Sensitive 1 (SOS1) is responsible for the efflux of Na from the cytoplasm, an important determinant of salt resistance in plants.. In this study, an ortholog of SOS1, referred to as NsSOS1, was cloned from Nitraria sibirica, a typical halophyte that grows in deserts and saline-alkaline land, and its expression and function in regulating the salt tolerance of forest trees were evaluated.. The expression level of NsSOS1 was higher in leaves than in roots and stems of N. sibirica, and its expression was upregulated under salt stress.

    Gene Expression:

    Article Title: X-ray-activated nanoscintillators integrated with tumor-associated neutrophils polarization for improved radiotherapy in metastatic colorectal cancer.
    Article Snippet: Radiotherapy, employing high-energy rays to precisely target and eradicate tumor cells, plays a pivotal role in the treatment of various malignancies.. Despite its therapeutic potential, the effectiveness of radiotherapy is hindered by the tumor’s inherent low radiosensitivity and the immunosuppressive microenvironment.. Here we present an innovative approach that integrates peroxynitrite (ONOO )-mediated radiosensitization with the tumor-associated neutrophils (TANs) polarization for the reversal of immunosuppressive tumor microenvironment (TME), greatly amplifying the potency of radiotherapy.



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    FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase <t>RNA</t> and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome <t>sequencing.</t>
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    FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase <t>RNA</t> and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome <t>sequencing.</t>
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    FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase <t>RNA</t> and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome <t>sequencing.</t>
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    FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase RNA and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome sequencing.

    Journal: The Journal of Biological Chemistry

    Article Title: Fragile histidine triad (FHIT) suppresses gastric cancer via translational regulation of the epigenetic modifier KDM6B

    doi: 10.1016/j.jbc.2026.111404

    Figure Lengend Snippet: FHIT regulates the downstream target KDM6B by mediating its mRNA translation. A , Circos plot displaying Gene Ontology (GO) terms enriched among differentially translated genes identified by Ribo-Seq data. The “LogFC” scale represents the normalized enrichment significance of each pathway (range, 0–1). B , polysome gradient traces from AGS and FHIT-overexpressing AGS cells separated on a 10% to 50% linear sucrose gradient. Western blot analysis confirms FHIT protein expression in both cells. C , RT–qPCR analysis of KDM6B mRNA distribution across polysome fractions in AGS cells with or without FHIT overexpression. mRNA levels were normalized to spiked luciferase RNA and plotted as the percentage of total mRNA in each gradient fraction relative to the input. D – F , RT–qPCR analysis of KDM6B mRNA expression and Western blot detection of KDM6B protein levels in gastric cancer cells or NOC-transformed cells with or without FHIT overexpression. G , Western blot analysis of KDM6B protein levels in NOC-transformed cells (GES-1-MNU) transfected with empty vector (EV) or FHIT-overexpressing constructs after treatment with the transcriptional inhibitor ActD to block nascent mRNA synthesis. The analyses were repeated three times, and the results were expressed as mean ± SD. ns, not significant, ∗ p < 0.05, and ∗∗ p < 0.01. ActD, actinomycin D; FHIT, fragile histidine triad; KDM6B, lysine-specific demethylase 6B; MNU, N -nitroso- N -methylurea; qPCR, quantitative PCR; Ribo-Seq, ribosome sequencing.

    Article Snippet: RNA transcriptome sequencing was then performed on each sample by Novogene Corporation.

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Over Expression, Luciferase, Transformation Assay, Transfection, Plasmid Preparation, Construct, Blocking Assay, Real-time Polymerase Chain Reaction, Sequencing